HGM 2011 - Dubai, UAE
Kai Q Lao - Abstract
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Kai Q Lao
Title: DEVELOPMENT AND APPLICATIONS OF SINGLE CELL RNA-SEQ ANALYSIS |
Dissecting the relationship between genotype and phenotype is one of the central goals in biology and medicine. Cell development is driven and controlled by temporal and spatial changes in gene transcription, followed by translation of the resulting messenger RNAs into proteins. Recent advances in high throughput sequencing technology make it possible to obtain information on single cell transcriptomes at high resolution by RNA-Seq analysis, which can be instructive concerning how individual cells respond to signals and other environmental cues at critical stages of cell fate determination, or when they acquire aberrant phenotype.all cells within an individual organism share a virtually identical genotype, but the individual transcriptomes reflect expression of a subset of genes, which is determined by their epigenetic state, including DNA methylation and histone modifications. Diverse cell types exhibit a unique transcriptome, which can be used to assess gene regulation network underlying their physiological functions, behavior and phenotype during development in multi-cellular organisms. Combined with live cell imaging and lineage tracing, it will be possible to decipher the full gene expression network underlying physiological functions of individual cells in embryos and adults, and for the analysis of diseases. |
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Sheikh Hamdan Bin Rashid Al Maktoum |
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Human Genome Organisation |
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Centre for Arab Genomic Studies |
