HGM 2011 - Dubai, UAE
BING REN - Abstract
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Bing Ren
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Eukaryotic gene transcription depends on the interplay between transcription factors and the cis-regulatory elements in the genome. While most of the protein coding sequences in the mammalian genome have been found, the cis-regulatory sequences that control their tissue- and temporal-specific expression remain largely unknown.report the first comprehensive survey of cis-regulatory sequences in a mammalian genome.use a high throughput experimental strategy to identify transcription factor binding and chromatin modification profiles in a large panel of mouse tissues and primary cells. By analyzing the genomic binding sites of RNA polymerase II, co-activator protein p300, insulator protein CTCF, and two chromatin modification marks H3K4me3 and H3K4me1, we identified over 270,000 cis regulatory sequences including promoters, enhancers and insulator sequences in the mouse genome. This map of cis-regulatory sequences provide functional annotation for70% of all the conserved non-coding regions previously identified in the mouse genome.identifies long-range enhancers for thousands of tissue-specifically expressed genes. It also exhibits a high level of conservation in the cis-regulatory elements between the human and mouse genomes, providing a powerful tool for the annotation of functional elements in the human genome. |
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Sheikh Hamdan Bin Rashid Al Maktoum |
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Human Genome Organisation |
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Centre for Arab Genomic Studies |
